LC3100 high performance liquid chromatography (HPLC)

HPLC is an instrument that applies the principles of high-performance liquid chromatography and is mainly used to analyze organic compounds with high boiling points, non-volatile, thermally unstable, and large molecular weights. It consists of a reservoir, pump, sample injector, chromatographic column, detector, recorder and other parts. The mobile phase in the reservoir is pumped into the system by the high-pressure pump, and the sample solution enters the mobile phase through the sampler, and is loaded into the chromatographic column (stationary phase) by the mobile phase. With different distribution coefficients, when the two phases move relative to each other, after repeated adsorption-desorption distribution processes, each component has a large difference in moving speed, and is separated into individual components and flowed out of the column in turn. When passing through the detector, the sample concentration is converted into an electrical signal and sent to the recorder, and the data is printed out in the form of a chromatogram.