There are many methods for the detection of formic acid. This article mainly introduces the determination of formic acid in food by ion chromatography.
While enjoying the food, pay more attention to food safety, pay attention to the dynamics of food safety, and ensure good health. So, do you know what ingredients are in the food we eat every day, and are these ingredients harmful to the human body?
Today we will talk about formic acid, an ingredient that may be contained in certain foods. What exactly is formic acid?
Formic acid, also known as formic acid, is often required to be tested as a functional ingredient in health products using ants as raw materials. At the same time, formic acid can be used as a disinfectant and preservative, and used as a flavor in beverages, candy and other foods. But formic acid is a strong organic acid and has certain toxicity. The concentration should not be too high, otherwise it will increase imported irritation and astringency, and strong irritating smell. FEMA stipulates the maximum residue limit of formic acid in soft drinks, candies, cold drinks, and roasted foods. Among them, soft drinks are 1.0 mg/kg, cold drinks are 5.0-18 mg/kg, and roasted foods are 5.0-6.1 mg/kg.

Chromatographic conditions | |
Column | Anion chromatography column, 4X250mm (4x10mm guard column) |
Eluent | Automatic eluent generator cartridge, OH-type |
Suppressor | Anion suppressor, suppressor current: 150mA |
Column temperature | 30℃ |
Detector | Conductivity detector, the temperature of the detection cell is 35℃ |
Flow rate | 1.0mL/min |
Injection volume | 100μL |
Gradient elution program | ||
Time(min) | Linear type | OH eluent concentration (mM) |
0 | Linear | 1 |
22 | Linear | 1 |
22.1 | Linear | 50 |
27 | Linear | 50 |
27.1 | Linear | 1 |
35 | Linear | 1 |
Standards and lower limit of determination
Standard 0.4mg/L formic acid standard chromatogram:

The lower limit of standard determination (0.05mg/L) chromatogram:

Group name | keep time | Peak area | Peak height | Theoretical plate number | Tailing factor | Signal-to-noise ratio |
Formic acid | 20.474 | 2.230 | 0.084 | 12793 | 1.025 | 73.312 |
Chromatograms detected by different samples

mongo juice chromatogram
