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Determination of formic acid in food by ion chromatography

There are many methods for the detection of formic acid. This article mainly introduces the determination of formic acid in food by ion chromatography.

While enjoying the food, pay more attention to food safety, pay attention to the dynamics of food safety, and ensure good health. So, do you know what ingredients are in the food we eat every day, and are these ingredients harmful to the human body?

Today we will talk about formic acid, an ingredient that may be contained in certain foods. What exactly is formic acid?
Formic acid, also known as formic acid, is often required to be tested as a functional ingredient in health products using ants as raw materials. At the same time, formic acid can be used as a disinfectant and preservative, and used as a flavor in beverages, candy and other foods. But formic acid is a strong organic acid and has certain toxicity. The concentration should not be too high, otherwise it will increase imported irritation and astringency, and strong irritating smell. FEMA stipulates the maximum residue limit of formic acid in soft drinks, candies, cold drinks, and roasted foods. Among them, soft drinks are 1.0 mg/kg, cold drinks are 5.0-18 mg/kg, and roasted foods are 5.0-6.1 mg/kg.

Chromatographic conditions
Column Anion chromatography column, 4X250mm (4x10mm guard column)
Eluent Automatic eluent generator cartridge, OH-type
Suppressor Anion suppressor, suppressor current: 150mA
Column temperature 30℃
Detector Conductivity detector, the temperature of the detection cell is 35℃
Flow rate 1.0mL/min
Injection volume 100μL
Gradient elution program
Time(min) Linear type OH eluent concentration (mM)
0 Linear 1
22 Linear 1
22.1 Linear 50
27 Linear 50
27.1 Linear 1
35 Linear 1

Standards and lower limit of determination

Standard 0.4mg/L formic acid standard chromatogram:

The lower limit of standard determination (0.05mg/L) chromatogram:

Group name keep time Peak area Peak height Theoretical plate number Tailing factor Signal-to-noise ratio
Formic acid 20.474 2.230 0.084 12793 1.025 73.312

Chromatograms detected by different samples

mongo juice chromatogram

wine chromatogram



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