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HPLC Detection of Peach Kernel Formula Granules

Peach kernel formula granules refer to formula granules that are processed and processed according to the main quality indicators of standard decoctions. This article mainly shares the detection method of Wanyi High Performance Liquid Chromatography on the characteristic map of peach kernel formula particles.

Equipment required for chromatographic testing

  • High performance liquid chromatograph: LC3200
  • Infusion pump: P3200Q
  • Column thermostat: CT3200
  • Autosampler: AS3200
  • Detector: DAD3200
LC3200 high performance liquid chromatography

Chromatographic conditions

Chromatographic column 18,4.6X250mm,5μm
flow rate 1.0ml/min Column temperature 25℃
Injection volume 10μL wavelength 214nm
Mobile phase mobile phase A:Acetonitrile mobile phase B:0.1% Phosphoric acid solution

Elution program

Time(min) A B
0 5 95
10 15 85
20 25 75
30 35 65
32 5 95
35 5 95

Pre-treatment of test samples

Take an appropriate amount of this product, grind finely, take about 0.2g, place it in a stoppered conical flask, add 25ml of 70% methanol, close the stopper, ultrasonic treatment (power 600W, frequency 40kHz) for 30 minutes, let cool, shake well, and filter , Take the continued filtrate.

Test chromatogram

Characteristic chromatogram of standard control in pharmacopoeia
Test characteristic chromatogram

Compare results

Group name Retention times relative retention time Relative retention time rating error
Peak 1 22.173 0.879 0.881 -0.22%
Tryptophan 22.99 0.911 0.901 1.16%
Amygdalin 25.223 1.000 1 0.00%
Peak 4 28.623 1.135 1.113 1.96%
Peak 5 29.473 1.168 1.144 2.14%

The relative retention time of the characteristic peaks all meet the standard requirements (take peak 3 amygdalin as the S peak, and calculate the relative retention time of each characteristic peak and the S peak, which should be within ±8% of the specified value).

Overlapping chromatograms of test samples

LC3200 High Performance Liquid Chromatography test results

Retention time Peak area
   Group No. Peak 1 Tryptophan Amygdalin Peak 4 Peak 5    Group No. Peak 1 Tryptophan Amygdalin Peak 4 Peak 5
1 22.073 22.84 25.14 28.523 29.406 1 504.425 312.277 5358.088 85.944 110.236
2 22.106 22.84 25.14 28.523 29.39 2 507.403 318.986 5287.775 83.977 109.466
3 22.09 22.823 25.156 28.523 29.406 3 506.295 319.06 5308.092 86.878 112.636
4 22.106 22.823 25.156 28.506 29.373 4 506.668 317.769 5318.533 85.182 113.882
5 2.056 22.79 25.106 28.473 29.356 5 508.056 323.707 5332.979 85.976 112.392
6 22.056 22.773 25.14 28.506 29.373 6 501.276 314.443 5266.563 84.615 108.537
7 22.106 22.806 25.156 28.506 29.39 7 505.455 320.333 5287.685 82.643 110.871
Average 22.085 22.814 25.142 28.509 29.385 Average 505.654 318.082 5308.531 85.031 111.146
RSD 0.10% 0.11% 0.07% 0.06% 0.06% RSD 0.45% 1.19% 0.59% 1.675 1.72%

From the above results, it can be seen that the high performance liquid chromatograph of Wanyi has excellent performance in the detection of the characteristic map of peach kernel formula particles, with good separation performance and excellent repeatability, and fully meets the requirements of the standard.

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