How to detect epinephrine by HPLC?
Adrenaline is a hormone and neurotransmitter released by the adrenal glands. Adrenaline will increase the contractility of the heart, dilate the blood vessels of the heart, liver, muscles and bones, and constrict the blood vessels of the skin and mucous membranes. It is a necessary product to save dying people or animals. Its biosynthesis is mainly to first form norepinephrine in medullary chromium cells, and then further through the action of phenylethanolamine N-methyl transferase (PNMT) to make norepinephrine methyl The formation of adrenaline.
1,Analytical equipment required for adrenaline testing
High performance liquid chromatograph: LC3100;
Binary high pressure gradient pump: P3100B;
Column thermostat: CT3100;
Detector: ECD3100 (ampere detector)
|Column||Welch Ultimate XB-C18 4.6x150mm,5μm|
|Mobile phase||A: 5% glacial acetic acid buffer solution (pH value is 3.42, add EDTA disodium salt 0.15mmol/L, sodium heptane sulfonate 2.7mmol/L) B: methanol (A:B=95:5 premix)|
|Column temperature||30℃||Conductivity cell temperature||30℃|
|ECD mode||DC Ampere||Voltage||0.65V|
|Sampling Rate||10SPS||Injection volume||20μl|
|Compound name||Keep time||Peak area||Peak height||Symmetry||Theoretical plate number||concentration（mg/L)|
|Standard curve series concentration（mg/L)|